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Understanding Light Microscopy
Selected technical specs and performance details for the confocal instruments:
Scan size windows for the Zeiss 880, when using a 100x 1.57 NA objective:
LSM 880 Field of View (169.73 KB)
How the ideal z-step size is influenced, when changing the pinhole diameter for the 100x 1.57 NA objective:
LSM 880 Pinhole Size Effect on Z-Step Size (227.09 KB)
Ideal pixel and z-step size for the Nikon A1, when using a variety of objectives with a 1 AU pinhole:
Nikon A1 Pixel and Z-Step Size Per Objective at 1 AU Pinhole (36.32 KB)
How the ideal scan area for 1024 by 1024 pixel changes on the Nikon A1, when changing the pinhole:
Nikon A1 Pinhole Size Effect on Scan Area (246.9 KB)
Optical sectioning power of the Nikon A1 for various objectives and pinhole dimensions:
Nikon A1 Pinhole Size Effect on Optical Sectioning (253.27 KB)
